Gene name |
SPAC1705.02 |
Gene ID |
42/B02 |
Gene synonyms/obsolete |
SPAC1F2.02c |
Gene product |
human 4F5S
homologeukaryotic conserved protein |
Entry clone |
Cloned |
ORF length (unspliced) |
441 |
ORF length (spliced) |
192 |
Entry clone length |
441 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC1705.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTCGTAAGTTTTAAAC |
Rev primer name |
SPAC1705.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTTTTTCCTTTTGAGCCA |
Amino acid length |
63 |
Molecular weight |
6.9 |
Isoelectric point (calc.) |
11.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus>nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |