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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC12D12.07c
Gene ID 42/B04
Gene synonyms/obsolete trx2
Gene product putative thioredoxinputative thioredoxin; similar to Sc TRX1 and TRX2
Entry clone Cloned
ORF length (unspliced) 448
ORF length (spliced) 366
Entry clone length 448
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC12D12.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGATCTTTTGCTCTGCG
Rev primer name SPBC12D12.07.Rv
Rev primer SEQ AGAAAGCTGGGTATTCTTGATATTTTGCCAAC
Amino acid length 121
Molecular weight 13.7
Isoelectric point (calc.) 9.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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