Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC22A12.13
Gene ID 42/B07
Gene synonyms/obsolete
Gene product human downs syndrome critical region-like
Entry clone Cloned
ORF length (unspliced) 363
ORF length (spliced)
Entry clone length 363
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC22A12.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTAAAAGTTCCTACTTA
Rev primer name SPAC22A12.13.Rv
Rev primer SEQ AGAAAGCTGGGTAAGTTTCTTCCAGTTGTTGT
Amino acid length 120
Molecular weight 13.9
Isoelectric point (calc.) 4.7
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 2
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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