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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1071.04c
Gene ID 42/B12
Gene synonyms/obsolete
Gene product putative signal peptidase; signal peptidase complex
Entry clone Cloned
ORF length (unspliced) 504
ORF length (spliced)
Entry clone length 504
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC1071.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCAAAATATAACGTTTC
Rev primer name SPAC1071.04.Rv
Rev primer SEQ AGAAAGCTGGGTAGTTTTCAATTTGAGACAAG
Amino acid length 167
Molecular weight 19
Isoelectric point (calc.) 9.4
Signal SEQ
No. of transmembrane domain 2
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LKSYLKYLLPL
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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