| Gene name |
SPBC1289.05c |
| Gene ID |
42/C02 |
| Gene synonyms/obsolete |
vma10 |
| Gene product |
vacuolar ATP synthase
(subunit G); V-type ATPase (G subunit); vacuolar proton pump
component |
| Entry clone |
Cloned |
| ORF length (unspliced) |
520 |
| ORF length (spliced) |
327 |
| Entry clone length |
520 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
507G:A |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC1289.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTAATTTGTATTTTTAT |
| Rev primer name |
SPBC1289.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTTAACATTGCAAGTTATA |
| Amino acid length |
108 |
| Molecular weight |
12.1 |
| Isoelectric point (calc.) |
8.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |