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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC887.01
Gene ID 42/C05
Gene synonyms/obsolete
Gene product acireductone dioxygenase; ARD domain
Entry clone Cloned
ORF length (unspliced) 537
ORF length (spliced)
Entry clone length 537
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC887.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGAGCATACATTTTCCA
Rev primer name SPBC887.01.Rv
Rev primer SEQ AGAAAGCTGGGTAAACATAAACACTTTTTTTA
Amino acid length 178
Molecular weight 20.9
Isoelectric point (calc.) 5.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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