Gene name |
SPAP27G11.06c |
Gene ID |
42/D02 |
Gene synonyms/obsolete |
|
Gene product |
AP-1 Golgi-associated
clathrin assembly proteinAP-1adaptor complex; involved in
vesicle-mediated transport |
Entry clone |
Cloned |
ORF length (unspliced) |
599 |
ORF length (spliced) |
489 |
Entry clone length |
599 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAP27G11.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTATAAAGTTCTTTCT |
Rev primer name |
SPAP27G11.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACGCTTCTTCACGCTGCCC |
Amino acid length |
162 |
Molecular weight |
18.6 |
Isoelectric point (calc.) |
7.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LAKWFNTLSI |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |