Gene name |
SPAC1A6.06c |
Gene ID |
42/D08 |
Gene synonyms/obsolete |
meu31 |
Gene product |
meiotic expression
upregulated; no apparent orthologs |
Entry clone |
Cloned |
ORF length (unspliced) |
609 |
ORF length (spliced) |
558 |
Entry clone length |
609 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC1A6.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGTGCATAAGTGAAAT |
Rev primer name |
SPAC1A6.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATATACAAATTGATTTTTCA |
Amino acid length |
185 |
Molecular weight |
21.2 |
Isoelectric point (calc.) |
7.5 |
Signal SEQ |
|
No. of transmembrane domain |
4 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
Golgi; cytoplasmic
dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |