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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC21C3.17c
Gene ID 42/D10
Gene synonyms/obsolete
Gene product hypothetical protein; Sp specific families; possibly Sp specific; similar to Sp SPCP31B10.04
Entry clone Cloned#
ORF length (unspliced) 611
ORF length (spliced) 561
Entry clone length 611
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC21C3.17.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAATTCTTTCTAGGATC
Rev primer name SPBC21C3.17.Rv
Rev primer SEQ AGAAAGCTGGGTAATGCTTTCTATATGAAGAC
Amino acid length 186
Molecular weight 21.2
Isoelectric point (calc.) 9.9
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LSCCILIVLFL
Localization (YFP) vacuole
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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