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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC4B4.06
Gene ID 42/E06
Gene synonyms/obsolete
Gene product hypothetical protein; conserved protein; required for normal mating efficiency in yeast???
Entry clone Cloned
ORF length (unspliced) 627
ORF length (spliced) 528
Entry clone length 627
No. of intron 2
Sequence status Finished
Sequence results 10C:T
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC4B4.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCGTGTTCCATCCATTTA
Rev primer name SPBC4B4.06.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCTTTCAGTACCTTGAAC
Amino acid length 175
Molecular weight 20.8
Isoelectric point (calc.) 8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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