Gene name |
SPBC4B4.06 |
Gene ID |
42/E06 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
conserved protein; required for normal mating efficiency in
yeast??? |
Entry clone |
Cloned |
ORF length (unspliced) |
627 |
ORF length (spliced) |
528 |
Entry clone length |
627 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
10C:T |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC4B4.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCGTGTTCCATCCATTTA |
Rev primer name |
SPBC4B4.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGCTTTCAGTACCTTGAAC |
Amino acid length |
175 |
Molecular weight |
20.8 |
Isoelectric point (calc.) |
8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |