Gene name |
SPCC584.02 |
Gene ID |
42/E07 |
Gene synonyms/obsolete |
cuf2 |
Gene product |
putative metal-binding
regulatory protein; involved in copper homeostasis;
DNA-binding protein; copper fist; involved in transcriptional
regulation; similar to Sp SPAC31A2.11c (paralog) |
Entry clone |
Cloned |
ORF length (unspliced) |
628 |
ORF length (spliced) |
534 |
Entry clone length |
628 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC584.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATCATTATTGACGGTAA |
Rev primer name |
SPCC584.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGGGATTCCGGATATATCG |
Amino acid length |
177 |
Molecular weight |
20 |
Isoelectric point (calc.) |
8.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Zeiss |