Gene name |
SPAC25H1.05 |
Gene ID |
42/G02 |
Gene synonyms/obsolete |
meu29 |
Gene product |
meiotic expression
upregulated; no apparent orthologs |
Entry clone |
Cloned |
ORF length (unspliced) |
654 |
ORF length (spliced) |
|
Entry clone length |
654 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC25H1.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTTGTTGTCAAGACTGC |
Rev primer name |
SPAC25H1.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAACAGATTCATCCAACACG |
Amino acid length |
217 |
Molecular weight |
24.6 |
Isoelectric point (calc.) |
8.4 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
2 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
Golgi; vacuole |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |