Gene name |
SPAC630.11 |
Gene ID |
43/A05 |
Gene synonyms/obsolete |
|
Gene product |
involved in
intracellular protein transport; involved in late endosome to
vacuole trafficking |
Entry clone |
Cloned |
ORF length (unspliced) |
731 |
ORF length (spliced) |
369 |
Entry clone length |
731 |
No. of intron |
5 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC630.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCGACCTCAGGAAGTA |
Rev primer name |
SPAC630.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAATTCTTCTTCGTGTTGA |
Amino acid length |
122 |
Molecular weight |
13.4 |
Isoelectric point (calc.) |
5.6 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
3 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
Golgi; vacuole
membrane |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |