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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC630.11
Gene ID 43/A05
Gene synonyms/obsolete
Gene product involved in intracellular protein transport; involved in late endosome to vacuole trafficking
Entry clone Cloned
ORF length (unspliced) 731
ORF length (spliced) 369
Entry clone length 731
No. of intron 5
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC630.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCCGACCTCAGGAAGTA
Rev primer name SPAC630.11.Rv
Rev primer SEQ AGAAAGCTGGGTAAAATTCTTCTTCGTGTTGA
Amino acid length 122
Molecular weight 13.4
Isoelectric point (calc.) 5.6
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 3
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) Golgi; vacuole membrane
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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