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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC216.03
Gene ID 43/A11
Gene synonyms/obsolete
Gene product conserved hypothetical; conserved hypothetical protein
Entry clone Cloned
ORF length (unspliced) 744
ORF length (spliced)
Entry clone length 744
No. of intron 0
Sequence status Finished
Sequence results 678G:A
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC216.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTGTTTTTGTTGTTTT
Rev primer name SPBC216.03.Rv
Rev primer SEQ AGAAAGCTGGGTATACTGAATAAGTATAGGAT
Amino acid length 247
Molecular weight 27.2
Isoelectric point (calc.) 6.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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