Gene name |
SPBC17D1.04 |
Gene ID |
43/B05 |
Gene synonyms/obsolete |
|
Gene product |
sequence orphan;
hypothetical protein |
Entry clone |
Cloned |
ORF length (unspliced) |
753 |
ORF length (spliced) |
|
Entry clone length |
753 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC17D1.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATGATTCTGAGGAAGA |
Rev primer name |
SPBC17D1.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATCCTCAAATTCTATCGAG |
Amino acid length |
250 |
Molecular weight |
28.7 |
Isoelectric point (calc.) |
5.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRYLVERLQI |
Localization (YFP) |
cytosol; SPB? |
Comments for localization |
SPB? |
Effect of LMB on protein
localization |
changed to:
nucleus>cytosol |
Microscope used for
observation |
DeltaVision |