| Gene name |
SPBC21B10.10 |
| Gene ID |
43/C02 |
| Gene synonyms/obsolete |
rps402; rps4-2 |
| Gene product |
40S ribosomal protein
S4 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
789 |
| ORF length (spliced) |
|
| Entry clone length |
789 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC21B10.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTTAGAGGACCCAAAAA |
| Rev primer name |
SPBC21B10.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCAAGACCTTTGAGAGCA |
| Amino acid length |
262 |
| Molecular weight |
29.6 |
| Isoelectric point (calc.) |
10.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol>>nucleolus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
cytosol=nucleus |
| Microscope used for
observation |
DeltaVision |