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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC132.03
Gene ID 43/C05
Gene synonyms/obsolete
Gene product sequence orphan; hypothetical protein
Entry clone Cloned#
ORF length (unspliced) 791
ORF length (spliced) 531
Entry clone length 791
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC132.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAACCCCAAGCGATTGAG
Rev primer name SPCC132.03.Rv
Rev primer SEQ AGAAAGCTGGGTAGAAATGGAAAGGGGATTGT
Amino acid length 176
Molecular weight 20.2
Isoelectric point (calc.) 7.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 126
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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