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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC13G7.06
Gene ID 43/C07
Gene synonyms/obsolete
Gene product phosphoadenosine phosphosulfate reductase; phosphoadenylyl-sulfate reductase (thioredoxin) activity; involved in methionine metabolism
Entry clone Cloned
ORF length (unspliced) 801
ORF length (spliced)
Entry clone length 801
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC13G7.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGCTCAATCGATACGCC
Rev primer name SPAC13G7.06.Rv
Rev primer SEQ AGAAAGCTGGGTACTGATCAGCCGTCTCCTTC
Amino acid length 266
Molecular weight 30.5
Isoelectric point (calc.) 7.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LHHFPETLDL
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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