| Gene name |
SPBC12D12.09 |
| Gene ID |
43/F03 |
| Gene synonyms/obsolete |
SPAC12D12.09 |
| Gene product |
Mad2-like protein;
possible mitotic checkpoint |
| Entry clone |
Cloned |
| ORF length (unspliced) |
933 |
| ORF length (spliced) |
624 |
| Entry clone length |
933 |
| No. of intron |
5 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC12D12.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAAACGACACAGGATG |
| Rev primer name |
SPAC12D12.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCCGAATTTGTATATTTC |
| Amino acid length |
207 |
| Molecular weight |
24.2 |
| Isoelectric point (calc.) |
4.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLNITNGLPL |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |