Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC17A2.03c
Gene ID 43/G04
Gene synonyms/obsolete vma6
Gene product vacuolar ATP synthase; V-type ATPase (D subunit); vacuolar proton pump component; V0 sector
Entry clone Cloned
ORF length (unspliced) 1075
ORF length (spliced) 1032
Entry clone length 1075
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC17A2.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATGCTCTGAGTTTTAA
Rev primer name SPAC17A2.03.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGAATTGGAACAATGTTC
Amino acid length 343
Molecular weight 39.3
Isoelectric point (calc.) 4.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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