Gene name |
SPBP4H10.20 |
Gene ID |
43/H04 |
Gene synonyms/obsolete |
nhm1; DcpS |
Gene product |
eukaryotic conserved
protein; HIT family of pyrophosphatase; histidine triad (HIT)
motif protein; co-purifies with the cap-binding complex eIF4F;
RNA-binding protein; predicted novel decapping pathway;
putative scavenger mRNA decapping enzyme; homology to the
human tumour suppressor Fhit; possibly associates with
exonuclease exosome components |
Entry clone |
Cloned |
ORF length (unspliced) |
1144 |
ORF length (spliced) |
915 |
Entry clone length |
1144 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBP4H10.20.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAGAAAGTTCTGCCGC |
Rev primer name |
SPBP4H10.20.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGCATTCATATTAGTTAAC |
Amino acid length |
304 |
Molecular weight |
35.1 |
Isoelectric point (calc.) |
6.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |