Gene name |
SPBC1711.11 |
Gene ID |
43/H09 |
Gene synonyms/obsolete |
|
Gene product |
sorting nexin; PX
domain; phosphoinositide binding; involved in intracellular
protein transport; similar to Sp SPAC6F6.12 |
Entry clone |
Cloned |
ORF length (unspliced) |
1173 |
ORF length (spliced) |
|
Entry clone length |
1173 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC1711.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTGAAATGTACAATTAA |
Rev primer name |
SPBC1711.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTCCTTCACGGCATGCTGC |
Amino acid length |
390 |
Molecular weight |
45.2 |
Isoelectric point (calc.) |
6.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQLSFQELCI |
Localization (YFP) |
nucleus>=cytosol;
periphery at site of septum formation |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |