Gene name |
SPAC17C9.08 |
Gene ID |
44/A10 |
Gene synonyms/obsolete |
pnu1; nuc1 |
Gene product |
endonuclease; involved
in RNA degradation; involved in the decrease in cellular RNA
during nitrogen starvation (implicated) |
Entry clone |
Cloned |
ORF length (unspliced) |
1237 |
ORF length (spliced) |
1008 |
Entry clone length |
1237 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC17C9.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTAGTAATCTTATCAA |
Rev primer name |
SPAC17C9.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAATATTTTCATAATAAAGA |
Amino acid length |
335 |
Molecular weight |
38 |
Isoelectric point (calc.) |
8.7 |
Signal SEQ |
|
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
DeltaVision |