Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC139.04c
Gene ID 44/B12
Gene synonyms/obsolete
Gene product fructosyl amino acid oxidase; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1302
ORF length (spliced)
Entry clone length 1302
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC139.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGAGAACCATTGTCAT
Rev primer name SPAC139.04.Rv
Rev primer SEQ AGAAAGCTGGGTACTTGTCGTAAGTAAACTCT
Amino acid length 433
Molecular weight 48.9
Isoelectric point (calc.) 5.8
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization occasionally a few cytoplasmic dots
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.