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Schizosaccharomyces pombe Postgenome Database

Gene name SPAP8A3.03
Gene ID 44/C11
Gene synonyms/obsolete
Gene product ZIP zinc transporter domain
Entry clone Cloned
ORF length (unspliced) 1362
ORF length (spliced)
Entry clone length 1362
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAP8A3.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTTTTATTGCAACGGTT
Rev primer name SPAP8A3.03.Rv
Rev primer SEQ AGAAAGCTGGGTAAACATAATATAAAAAAGAA
Amino acid length 453
Molecular weight 50.2
Isoelectric point (calc.) 4.9
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 7
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LQRFFIYGLFL/LGVFPELLEI
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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