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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC3C7.12
Gene ID 44/D06
Gene synonyms/obsolete noc1; tip1
Gene product microtubule-associated protein; involved in microtubule based movement; similar to eukaryotic intermediate filament proteins; involved in actin cytoskeletal organization; involved in cell polarity; CLIP170 like protein; CAP-Gly domain
Entry clone Cloned
ORF length (unspliced) 1386
ORF length (spliced)
Entry clone length 1386
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC3C7.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTTCCTCTTGGCAGTGT
Rev primer name SPAC3C7.12.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCTTCGTCTGTGCTGCCA
Amino acid length 461
Molecular weight 52.6
Isoelectric point (calc.) 4.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LEHTIEQLTI
Localization (YFP) cytosol; cytoplasmic dots at cell tip
Comments for localization
Effect of LMB on protein localization changed to: intranuclear microtubule bundle (nucleus>>cytosol; cytoplasmic dots at cell tip)
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images
LMB 4 images) See all images

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