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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1773.13
Gene ID 44/F10
Gene synonyms/obsolete
Gene product aromatic aminotransferase; similar to Sp SPCC569.07 and SPAC56E4.03
Entry clone Cloned
ORF length (unspliced) 1446
ORF length (spliced)
Entry clone length 1446
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC1773.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATCCGGAATAGTGAGGA
Rev primer name SPBC1773.13.Rv
Rev primer SEQ AGAAAGCTGGGTAAGGGCAGATTCGTACTCTG
Amino acid length 481
Molecular weight 54.7
Isoelectric point (calc.) 7.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LKSLVPSLLSL
Localization (YFP) cytosol=nucleus
Comments for localization aggregates by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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