| Gene name |
SPAC2F3.16 |
| Gene ID |
44/G06 |
| Gene synonyms/obsolete |
|
| Gene product |
zinc-finger protein;
with possiblzinc finger protein; zf-C2H2 type(; zf-C3HC4 type
(RING finger); ubiquitin ligase (E3); zinc-binding protein;
LIM domain signature; no apparent Sc ortholog |
| Entry clone |
Cloned in 2006
trial |
| ORF length (unspliced) |
1464 |
| ORF length (spliced) |
1278 |
| Entry clone length |
1464 |
| No. of intron |
3 |
| Sequence status |
Partially
sequenced |
| Sequence results |
100% match in both
ends |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC2F3.16.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCATTGTCGGAGTATCT |
| Rev primer name |
SPAC2F3.16.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATATAAAACTGTGAATCCCG |
| Amino acid length |
425 |
| Molecular weight |
49.9 |
| Isoelectric point (calc.) |
6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no expression
clone |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
|