| Gene name |
SPBC25B2.11 |
| Gene ID |
44/G08 |
| Gene synonyms/obsolete |
pof2 |
| Gene product |
F-box protein;
leucine-rich repeat protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1475 |
| ORF length (spliced) |
1392 |
| Entry clone length |
1475 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC25B2.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCGGGTGCCTAATGAAGT |
| Rev primer name |
SPBC25B2.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATATTCTATTACGAATAAAT |
| Amino acid length |
463 |
| Molecular weight |
52.3 |
| Isoelectric point (calc.) |
8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LATGISRLNL/LSHFITFLNL/LDAMRALSL/LSQSLTTLHL |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |