| Gene name |
SPAC1A6.05c |
| Gene ID |
44/H09 |
| Gene synonyms/obsolete |
|
| Gene product |
phospholipase; patatin
domain |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1541 |
| ORF length (spliced) |
1452 |
| Entry clone length |
1541 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC1A6.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTAAAAATGAAATTAA |
| Rev primer name |
SPAC1A6.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTTAAACTTTTAGACCTT |
| Amino acid length |
483 |
| Molecular weight |
54.6 |
| Isoelectric point (calc.) |
7.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LERVMKLLI/LTFRLFAELGI |
| Localization (YFP) |
cytoplasmic dots;
cytosol; site of septum formation |
| Comments for localization |
aggregates by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |