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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1198.08
Gene ID 45/A05
Gene synonyms/obsolete
Gene product peptidase family M20
Entry clone Cloned
ORF length (unspliced) 1550
ORF length (spliced) 1425
Entry clone length 1550
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC1198.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTTTGGATAAGTTATA
Rev primer name SPBC1198.08.Rv
Rev primer SEQ AGAAAGCTGGGTAAGGAGAAACAGAAGCCAAC
Amino acid length 474
Molecular weight 52.5
Isoelectric point (calc.) 5.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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