| Gene name |
SPAC23H4.10c |
| Gene ID |
45/A09 |
| Gene synonyms/obsolete |
thi4 |
| Gene product |
bifunctional enzyme;
involved in thiamine biosynthesis; hydroxyethylthiazole
kinase; thiamine-phosphate dipyrophosphorylase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1557 |
| ORF length (spliced) |
|
| Entry clone length |
1557 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC23H4.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAACGTCAAATTGACTA |
| Rev primer name |
SPAC23H4.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCAGCTTTCTCTAATTTG |
| Amino acid length |
518 |
| Molecular weight |
55.5 |
| Isoelectric point (calc.) |
6.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol; cytoplasmic
dots |
| Comments for localization |
aggregates by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |