| Gene name |
SPCC553.12c |
| Gene ID |
45/B03 |
| Gene synonyms/obsolete |
SPCC794.13 |
| Gene product |
low similarity to NADH
dehydrogenase subunit 2 by multiple alignment; no apparent Sc
ortholog |
| Entry clone |
Cloned in 2006
trial |
| ORF length (unspliced) |
1566 |
| ORF length (spliced) |
|
| Entry clone length |
1566 |
| No. of intron |
0 |
| Sequence status |
Partially
sequenced |
| Sequence results |
100% match in both
ends |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC553.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGGATAGTTTTGATGC |
| Rev primer name |
SPCC553.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAAGTGAAGACTTATTATA |
| Amino acid length |
521 |
| Molecular weight |
60.5 |
| Isoelectric point (calc.) |
8.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
10 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSNFVLRNLML |
| Localization (YFP) |
no expression
clone |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
|