| Gene name |
SPCC1906.03 |
| Gene ID |
45/B08 |
| Gene synonyms/obsolete |
wtf19; wtf1 |
| Gene product |
wtf element |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1572 |
| ORF length (spliced) |
1182 |
| Entry clone length |
1572 |
| No. of intron |
5 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC1906.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGAATAAATATTATCC |
| Rev primer name |
SPCC1906.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGACCTCGCTTTCAACTTCC |
| Amino acid length |
393 |
| Molecular weight |
43.9 |
| Isoelectric point (calc.) |
5.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
8 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFIMGNVLFL |
| Localization (YFP) |
Golgi; cytoplasmic
dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |