| Gene name |
SPAC17A2.01 |
| Gene ID |
45/C01 |
| Gene synonyms/obsolete |
car1; sod1;
SPAC1B1.05 |
| Gene product |
MFS efflux
transporter; repressed by thiamine; deletion mutant resistant
to amiloride; similar to Sp SPCC576.17C and SPCC965.13 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1581 |
| ORF length (spliced) |
|
| Entry clone length |
1581 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC17A2.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCCAGTAAAATTGCGAG |
| Rev primer name |
SPAC17A2.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTTGTTTTCGAGAGGTAAA |
| Amino acid length |
526 |
| Molecular weight |
58.4 |
| Isoelectric point (calc.) |
7.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
11 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
periphery |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |