| Gene name |
SPCC16C4.01 |
| Gene ID |
45/C03 |
| Gene synonyms/obsolete |
SPCC5E4.09 |
| Gene product |
Sad1-interacting
factor 2; DUF155; similar to S. cerevisiae YDL001W; 2
predicted transmembrane helices |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1582 |
| ORF length (spliced) |
1341 |
| Entry clone length |
1582 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC16C4.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTAATAGAATTGGGCC |
| Rev primer name |
SPCC16C4.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACTCGATAAATATGGTTTA |
| Amino acid length |
446 |
| Molecular weight |
51.8 |
| Isoelectric point (calc.) |
5 |
| Signal SEQ |
|
| No. of transmembrane domain |
2 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Leica |