Gene name |
SPAC30C2.08 |
Gene ID |
45/D02 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; hypothetical protein; surface protein adhesion
molecule |
Entry clone |
Cloned |
ORF length (unspliced) |
1612 |
ORF length (spliced) |
1374 |
Entry clone length |
1612 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC30C2.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATGTCTTATGAGCCAGC |
Rev primer name |
SPAC30C2.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATAAGTTTCCATCCGTGTTC |
Amino acid length |
457 |
Molecular weight |
52.6 |
Isoelectric point (calc.) |
5.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
367 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LEDCLHLLSL/LLNKLKHLTL |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |