Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC13G7.05
Gene ID 45/D03
Gene synonyms/obsolete
Gene product acyl-coA-sterol acyltransferase
Entry clone Cloned; Mixture
ORF length (unspliced) 1614
ORF length (spliced)
Entry clone length 1614
No. of intron 0
Sequence status Finished
Sequence results Mixture of 45/D03 and 45/D04
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC13G7.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACAGATACTCCTAGGAT
Rev primer name SPAC13G7.05.Rv
Rev primer SEQ AGAAAGCTGGGTAAAAGACAATGTATAAGATA
Amino acid length 537
Molecular weight 63
Isoelectric point (calc.) 9.4
Signal SEQ
No. of transmembrane domain 7
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LDFIGKLRI
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.