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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC36.03c
Gene ID 45/D06
Gene synonyms/obsolete
Gene product unknown specificity; transporter; similar to Sp SPAC11D3.05 and SPCC794.04C and SPCC569.05C and SPBC530.15C and SPBC36.01C and SPBC36.02C and SPBC530.02 and SPBC947.06C; involved in amine/polyamine transport; tandem duplication
Entry clone Cloned
ORF length (unspliced) 1617
ORF length (spliced)
Entry clone length 1617
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC36.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTATAACAATAATTCTTC
Rev primer name SPBC36.03.Rv
Rev primer SEQ AGAAAGCTGGGTACAAATTAACGGTATTTTTA
Amino acid length 538
Molecular weight 59.1
Isoelectric point (calc.) 7.2
Signal SEQ
No. of transmembrane domain 11
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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