Gene name |
SPAC1D4.03c |
Gene ID |
45/D11 |
Gene synonyms/obsolete |
|
Gene product |
involved in
intracellular protein transport; involved in protein-vacuolar
targeting; Sc deletion hypersensitive to brefeldin A or
monensin, two drugs that affect intracellular transport; SAND
family protein |
Entry clone |
Cloned# |
ORF length (unspliced) |
1628 |
ORF length (spliced) |
1542 |
Entry clone length |
1628 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
#check |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC1D4.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAACCAACATCTGAGCA |
Rev primer name |
SPAC1D4.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAACGAAAGGTTTGTTTGT |
Amino acid length |
513 |
Molecular weight |
58 |
Isoelectric point (calc.) |
6.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNAISPLPL |
Localization (YFP) |
periphery at site of
septum formation; nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |