Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1442.09
Gene ID 45/E03
Gene synonyms/obsolete trp3
Gene product anthranilate synthase (component I)
Entry clone Cloned
ORF length (unspliced) 1653
ORF length (spliced) 1470
Entry clone length 1653
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC1442.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAGGTAATTTGATTTTT
Rev primer name SPCC1442.09.Rv
Rev primer SEQ AGAAAGCTGGGTATGCTGAAGAGTTTTCTTCT
Amino acid length 489
Molecular weight 54.9
Isoelectric point (calc.) 5.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LTPSVAYLKL
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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