Gene name |
SPAC1D4.04 |
Gene ID |
45/E05 |
Gene synonyms/obsolete |
cct2 |
Gene product |
T-complex protein 1
(beta subunit); chaperonin-containing T-complex; involved in
protein folding; involved in actin folding; involved in
tubulin folding |
Entry clone |
Cloned |
ORF length (unspliced) |
1667 |
ORF length (spliced) |
1584 |
Entry clone length |
1667 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC1D4.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTAATATCATGTGTAAA |
Rev primer name |
SPAC1D4.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACATTCGTTCACGGGGTCTT |
Amino acid length |
527 |
Molecular weight |
56.6 |
Isoelectric point (calc.) |
5.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |