| Gene name |
SPCC1259.12c |
| Gene ID |
45/E12 |
| Gene synonyms/obsolete |
|
| Gene product |
SPRY domain; CTLH
domain; similar to human ranbpm; involved in vacuolar import
and degradation |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1695 |
| ORF length (spliced) |
1461 |
| Entry clone length |
1695 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC1259.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTAGTACACCAAAGAA |
| Rev primer name |
SPCC1259.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTTTTAAATAAAAAAAAA |
| Amino acid length |
486 |
| Molecular weight |
55.6 |
| Isoelectric point (calc.) |
6.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRFLRFLQL |
| Localization (YFP) |
ambiguous
structure |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal |