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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC821.12
Gene ID 45/F06
Gene synonyms/obsolete orb6
Gene product serine/threonine protein kinase; Ndr kinase family; involved in cell polarity (maintenance) (required); involved in cell cycle regulation; involved in osmotic stress; essential; possibly functions downstream of Shk1p; interacts physically with Skb1p; Skb1p affects the localization of Orb6p
Entry clone Cloned
ORF length (unspliced) 1722
ORF length (spliced) 1410
Entry clone length 1722
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC821.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATAAGAATGATTACTT
Rev primer name SPAC821.12.Rv
Rev primer SEQ AGAAAGCTGGGTACAATGCTCCTTTCATCGTT
Amino acid length 469
Molecular weight 54.6
Isoelectric point (calc.) 7.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LYYAFQDSLYL
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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