| Gene name |
SPBC418.01c |
| Gene ID |
45/F09 |
| Gene synonyms/obsolete |
his4;
SPBC887.20c |
| Gene product |
bifunctional enzyme;
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)
methylideneamino]imidazole-4-carboxamide isomerase; involved
in histidine biosynthesis (4th step); glutamine
amidotransferase |
| Entry clone |
Cloned in 2004
trial |
| ORF length (unspliced) |
1732 |
| ORF length (spliced) |
1626 |
| Entry clone length |
1732 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC418.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTGTTTCAATAGTTGA |
| Rev primer name |
SPBC418.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGTGCGAACCAGAACGTCG |
| Amino acid length |
541 |
| Molecular weight |
59.3 |
| Isoelectric point (calc.) |
6.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |