Gene name |
SPBC1711.10c |
Gene ID |
45/G03 |
Gene synonyms/obsolete |
|
Gene product |
Cdc48-Ufd1-Npl4
complex component (putative); involved in ubiquitin-dependent
proteolysis; involved in proteasomal processing; involved in
the recognition of polyubiquitin-tagged proteins |
Entry clone |
Cloned |
ORF length (unspliced) |
1745 |
ORF length (spliced) |
1638 |
Entry clone length |
1745 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC1711.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTATGCAAAGCAATCGT |
Rev primer name |
SPBC1711.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGTACATATAAAAAAAATAA |
Amino acid length |
545 |
Molecular weight |
60.9 |
Isoelectric point (calc.) |
5.7 |
Signal SEQ |
|
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSNFSVLLAI |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal |