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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1711.10c
Gene ID 45/G03
Gene synonyms/obsolete
Gene product Cdc48-Ufd1-Npl4 complex component (putative); involved in ubiquitin-dependent proteolysis; involved in proteasomal processing; involved in the recognition of polyubiquitin-tagged proteins
Entry clone Cloned
ORF length (unspliced) 1745
ORF length (spliced) 1638
Entry clone length 1745
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC1711.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTATGCAAAGCAATCGT
Rev primer name SPBC1711.10.Rv
Rev primer SEQ AGAAAGCTGGGTAGTACATATAAAAAAAATAA
Amino acid length 545
Molecular weight 60.9
Isoelectric point (calc.) 5.7
Signal SEQ
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LSNFSVLLAI
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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