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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC29B12.14c
Gene ID 45/G04
Gene synonyms/obsolete
Gene product purine transporter; similar to Sp FUR4
Entry clone Cloned
ORF length (unspliced) 1746
ORF length (spliced)
Entry clone length 1746
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC29B12.14.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCAAAATGGAGATTGAC
Rev primer name SPAC29B12.14.Rv
Rev primer SEQ AGAAAGCTGGGTAGAATACAGAAGATTCTTTG
Amino acid length 581
Molecular weight 64.1
Isoelectric point (calc.) 7.4
Signal SEQ
No. of transmembrane domain 11
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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