| Gene name |
SPCC1494.06c |
| Gene ID |
45/H05 |
| Gene synonyms/obsolete |
|
| Gene product |
DEAD/DEAH box
helicase; ATP-dependent; RNA helicase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1788 |
| ORF length (spliced) |
|
| Entry clone length |
1788 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC1494.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAAAATCTGGTGGAAT |
| Rev primer name |
SPCC1494.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGATTTACGCTTCATAGAG |
| Amino acid length |
595 |
| Molecular weight |
67.4 |
| Isoelectric point (calc.) |
9.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
372 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIPILELLL/LDKLKFLVI/LLAYILLKLRL |
| Localization (YFP) |
nucleolus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleolus>nucleus |
| Microscope used for
observation |
Confocal |