Gene name |
SPAC31A2.12 |
Gene ID |
45/H06 |
Gene synonyms/obsolete |
|
Gene product |
PY motif protein;
involved in heavy metal resistance; similar to Sp SPCC584.15C
(paralog); possibly binds to ubiquitin-protein ligase |
Entry clone |
Cloned |
ORF length (unspliced) |
1791 |
ORF length (spliced) |
|
Entry clone length |
1791 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC31A2.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATATTCTCTCAAGGAA |
Rev primer name |
SPAC31A2.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATTTCTTCTTGAAAATCCA |
Amino acid length |
596 |
Molecular weight |
65.9 |
Isoelectric point (calc.) |
8.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVPLLKRLTI/LRAALPVVLMI/LIAGLTELAL |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
nucleus>cytosol |
Microscope used for
observation |
Confocal |