Gene name |
SPCC1223.07c |
Gene ID |
45/H07 |
Gene synonyms/obsolete |
|
Gene product |
aspartate-tRNA ligase;
involved in aspartyl-tRNA aminoacylation; aspartate-tRNA
ligase activity |
Entry clone |
Cloned |
ORF length (unspliced) |
1801 |
ORF length (spliced) |
1743 |
Entry clone length |
1801 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
599A:addition |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC1223.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGAGATGGAAAAACA |
Rev primer name |
SPCC1223.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGGAAGCAAACGCTTTGGA |
Amino acid length |
580 |
Molecular weight |
66.6 |
Isoelectric point (calc.) |
6.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
48 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LERVVMFYLNL |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal |